Evaluation of Lithotriptic Activity of Mahaboobathi Parpam for Kalladiappu (Struvite Crystal Growth Inhibition Assay)

Abstract

Siddha medicine is originated from Tamil culture in Southern India. Worldwide, there are 75% of herbal plants are used for AYUSH and Modern medicines. Urolithiasis is a condition characterized by formation of stone in kidney,ureter, urinary bladder which is composed of calcium oxalate, cystine phosphate and uric acid. It has higher prevalence in men than women in both developed and developing countries, nearly 12% of the world populations are at potential risk of some stages of urolithiasis. Mahaboobathi parpam (MBP) is a Lithotriptic medicine made up of both mineral and animal origin which consists of Pavazha parpam,Vengaara parpam, Maankombu parpam ,Aamaiyottu parpam ,Muthuchippi parpam ,Karudakal parpam,Padikara parpam ,Sangu parpam are indicated for Kalladaippu, Neeradaippu,Sadhaiyadaippu, Neerkattu, Maarbu noi, Shayam, Eelai, Meghakaangai in the text of Sigicha Rathina Dheepam ennum Vaithiya Nool. The objective of the study is to evaluate the lithotriptic action of Mahaboobathi parpam. In-vitro Lithotriptic activity (Crystal Growth Inhibition assay) for Urolithiasis. Silica hydrogel matrix was divided in to two groups which were control, MBP treated groups. Test drug is screened at two dose levels of 0.5% and 1%. The efficiency of the formulation was screened by comparing the crystal size of the control and treatment medium. Average size of the crystal was higher in the control medium with the measured length of 2.1 ± 0.41. The size of the crystal was significantly decreased in medium contains 0.5% of test drug MP with the Avg length of 1.76 ± 0.27 cm. Similarly, the size of the crystal aggregates in medium contains 1 % of test drug MP was found to be 0.88 ± 0.42 cm. These study shows efficacy of lithotriptic action of Mahaboobathi parpam. Results states that the siddha formulation offers maximum percentage inhibition on the crystal growth in the tested medium, this efficacy may be due to presence of versatile constituents in MBP.