Preliminary Phytochemical Analysis and GC-MS Analysis of Polyherbal Siddha Formulation – Thathu Pushti Chooranam

Abstract

The primary objective of the study is to investigate the preliminary phytochemical studies of aqueous extracts and GC-MS analysis of the concentrated ethanol extract of “Thathu pushti chooranam” for fixing the parameters of pharmacognostical standard. Preliminary phytochemical analysis was done using standard procedure with aqueous extracts and the GC-MS analysis was performed using JEOL GC-Mate-11 with HP-5 capillary column with concentrated ethanol extracts of Thathu pushti chooranam. The results of the phytochemical analysis showed the presence of alkaloids, steroids, triterpenoids, coumarins, phenols and sugars. The results of GC-MS analysis showed the presence of components such as Lanosta-7,9(11)-dien-18-oic acid,22,25-epoxy-3beta,17,20-trihydroxy-gamma lactone, 1- Tetradecanamine, n-Hexadecylsuccinic anhydride, 1,1-Cyclobutanedicarboxamide, 4alpha,23,24-Trimethyl-5alpha-cholest-22-en-3beta-ol trimethylsilyl ether and 13,6 Octadeconoic acid. From the above analysis it was concluded that “Thathu pushti chooranam” shows the presence of several phytochemical components which possess the spermatogenesis and antioxidant activities. These findings were strongly suggestive of its therapeutic value. This study forms the basic foundation to further clinical research which will be followed by safety and efficacy studies later.