Evaluation of Invitro Anticarcinogenic Activity of Pirandai Uppu Using MTT Assay

Abstract

Introduction: The pirandai uppu (PU) is a simple formulation derived from ash of aerial parts of Cissus quadrangularis. Since documentation regarding therapeutic effects of PU is lacking we aimed in evaluating the anticancer activity of PU through MTT assay. Aim and Objective: The present study is aimed to 
evaluate the anticancer potential using in vitro assay system. Materials : MCF-7 cells (1 × 105 cells/ml) were seeded in the 96-well plate and incubated at 37˚C in CO2 incubator. After 24 hr of incubation, the plates were washed thrice with 0% media (no serum added) and Pirandai Uppu (PU) treated with following concentration - 5, 25, 50, 75, 100μg with 10% DMEM medium.  After incubation, media was carefully removed and DMSO were added in plate for the purple color formation, which is directly proportional to the number of viable cells. The 96 plate was subjected to OD value at 570 nm using a microplate reader ( The percentage of cell viability was calculated. Results: From the results, it is evident that the prepared PirandaiUppu (PU) enormously regulate the cancer cell viability . 5, 25 and 50μg of PU was regulate the cell proliferation upto 25-30% than control. Meanwhile 100μg of PU negatively influence the cell proliferation on the whole. Notably, the 5μg and 25μgPU will be the effective target for cancer treatment . Conclusion: Thus Pirandai uppu can be used as an effective anticancer drug in addition to anti inflammatory.