Evaluation of in vitro Anti-Inflammatory Activity of Pirandai Uppu Using Inhibition of Protein Denaturation Method

Abstract

Background: Pirandai uppu (salt derived from Cissus quadrangularis) is used in the treatment of ailments like hemorrhoids and dysmenorrhea. Inflammatory  reactions are evident in both cases. Prostaglandin plays significant role in the pathomechanism of Dysmenorrhea. It is responsible for the generation of an  inflammatory response. Several inflammatory markers such as prostaglandin F2 alpha, interleukin-6, and vasopressin are involved in Dysmenorrhea. Inflammatory markers such as PGF2α, IL- 6, vasopressin, VEGF are found in higher concentrations in case of Dysmenorrhea. Hemorrhoidal specimens are  found to have a severe inflammatory reaction involving the vascular wall and surrounding connective tissue. This inflammatory process results in the vulnerability of the arterioles causing erosion during defecation causing hemorrhage. Pirandai uppu is effective in the treatment of such conditions involving  inflammatory pathways. Hence, strongly suggesting that it could have potent anti-inflammatory activity. Objective: To prove the In vitro anti- inflammatory  activity of Pirandai uppu by Inhibition of albumin denaturation and Inhibition of protein denaturation method. Method: The invitro anti-inflammatory assay consists of assaying the effect of extracts of Pirandai uppu against protein denaturation of protein (egg albumin and serum albumin) and measuring the absorbance. The percentage inhibition of protein denaturation is calculated and compared with the obtained result of Standard -Diclofenac sodium.  Results  and Conclusion: Based on the results of the percentage of Inhibition of denaturation of protein obtained by calculations using the absorbance it is concluded that Pirandai uppu has good anti-inflammatory activity. The percentage inhibition of 200μg/mL of Pirandai uppu obtained by egg albumin method and bovine  serum albumin method was found to be 3.8 and 1.22 times respectively greater than that of 200μg/mL aqueous stem extract of Cissus quadrangularis.It can  be concluded that the process of deriving concentrated salts from medicinal herbs devised by Siddhars is of immense scientific significance. As denaturation of  tissue protein is one of the causes of inflammatory and arthritic diseases the results of our study reveal that Pirandai uppu exerts potent anti-inflammatory  activity and can be used to effectively treat inflammatory and arthritic conditions.